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The IPGs were first equilibrated in a buffer of 75 mM TrisHCl (pH 8.8), 29.3% glycerol, 2% SDS, 6 M urea, 0.002% bromophenol blue, and 1% DTT for 15 min and then agitated again for 15 min with the same buffer, but instead of DTT with 2.5% iodoacetamide (IAA)

Similarly, animal studies have demonstrated that while low doses of RSV promote the healing of indomethacin-induced gastric ulcers, higher doses exacerbate ulcer severity and delay the healing of pre-existing ulcers (57)

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