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The plate was incubated at room temperature for 510 min, before luminescence was measured using a Victor 3 1420 Multilabel Counter (Wallac 1420 Workstation, version 3.00 revision 5

Green recommends following a specific skincare routine for several days afterward to ensure optimal rejuvenation results and prevent irritation

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References: Seiwerth, S., Milavic, M., Vukojevic, J., Gojkovic, S., Krezic, I., Vuletic, L

All animals (up to 5 mice per cage) were housed under controlled conditions in ventilated cages, including a 12-h light/dark cycle, temperatures of 22 4 C or 21 2 C, and relative humidity ranges of 50 15% or 3070% at The Jackson Laboratory and Oslo University Hospital Rikshospitalet, respectively