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After blocking, the membranes were incubated with primary and secondary HRP-conjugated antibodies and then visualized with a ChemiDoc system (Bio-Rad)

Garlic fluid extracts (aged garlic extract) When garlic cloves are incubated in a solution of ethanol and water for up to 20 months, allicin is mainly converted to allyl sulfides, which are lost by evaporation or converted to other compounds (138)

Research Focus: Investigation into metabolic regulation, glycemic control, and dual-incretin receptor pathways

Total cellular extracts (200 g of protein) were incubated with an anti-IKK- antibody (1 g/mL) in IP buffer overnight at 4 C on a rocker