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NanoBiT assays were carried out as previously described (28): 24 hours after transfection, cells were incubated in 3 mM glucose for a minimum of 2 hours, detached and resuspended in NanoGlo Live cell substrate (N2012, Promega), 1:20 dilution in HBSS, and seeded onto an opaque white 96-well half-area plate
Therefore, not adjusting fasting times or limiting only a certain group of patients to extended liquid fasting might lead to aspiration events in asymptomatic patients who follow the standard recommendations (8 h for solids, 2 h for clear liquids)
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You can press a clean cotton ball to the area until the bleeding stops
