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For saturation binding assays, cell lysates (100 L, 25 g of protein for HEK-293 cells and 1020 g of protein for INS-1E cells, quantified by the Bradford Assay) were incubated with the indicated concentrations of [ 3 H]RX821002 (90 min, room temperature)

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Under this condition, almost all SCs in neighboring control HFs were tdTomato positive, while the de novo formed SCs in the ablated HFs were all tdTomato negative (Figure 2E)

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