For peptide reconstitution, the bacteriostatic water should be added slowly to the lyophilized peptide vial, directed against the glass wall rather than directly onto the peptide cake
The chloroform layer was separated, washed with water (0.3 L), dried over magnesium sulphate, and evaporated to dryness
However, even this method is not able to resolve every individual sample, e.g
Patients classically present with abdominal pain, hepatomegaly, and ascites
Indeed, both CBS and CSE can synthesise cysteine persulphide (CysSSH) and poly-sulphides (CysS ( n ) SH) from cystine, 104,136 whereas 3-MP-activated 3-MST can generate from cysteine, glutathione or n -acetylcysteine, the corresponding per-sulphides (respectively, CysSSH, GSSH and NACSSH)