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The process of mitochondrial fusion involves the exchange of mtDNA, intermediates of the tricarboxylic acid cycle (TCA cycle), and respiration-related proteins

Push the syringe needle into the powder vial at an angle so that the water runs down the glass wall, not directly onto the powder cake

Each wash should also be analyzed to observe any eluted protein, and bait- and prey-free controls should also be used to confirm that there are no bait-prey interactions in the bait lysate and that the prey protein does not bind to the immobilized support

Sequencing was carried out by Sanger methodology on ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA) and 100% concordance with restriction fragment length polymorphism assays was shown providing confidence in using the full dataset in subsequent analysis

10.1007/s13300-021-01113-y Diabetes Ther