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Relative level of ROS was detected centrifuging liver tissue in the appropriate buffer (20 mmol/L Tir-HCl (pH 7.4), 20 mmol/L NaH 2 PO 4 , 5 mmol/L MgCl 2 , 130 mmol/L KCl and 30 mmol/L glucose) and incubating the supernatant with DCFH-DA (2, 7-dichlorodihydrofluorescein diacetate) for 15 min at 37 C

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This panel was validated across multiple age groups ranging from 1 to 13 years old, all showing relatively high AUCs of 0.870.91 [195]

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Food Sources of Collagen and Glutathione Both glutathione and collagen are produced within the body