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DNA constructs encoding ligands with C-terminal Twin-Strep tags were synthesized (Twist Bioscience), assembled using NEBuilder HiFi DNA Assembly, and validated using capillary electrophoresis and Qubit fluorometry

A polymeric (big molecule from repeated subunits) emulsifier that comes from Inulin, a naturally occurring polysaccharide found in the roots and rhizomes of several plants

AMPK agonists inhibit ferroptosis by activating AMPK, which subsequently upregulates GPX4 expression and downregulates lipocalin-2 expression in vivo (63)

The safety concern patients ask about most: liver stress

Smith KE, Borden LA, Wang CH, Hartig PR, Branchek TA, Weinshank RL: Cloning and expression of a high affinity taurine transporter from rat brain