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Step 6: Refrigerate immediately at 2-8C (36-46F)

On the experimental day, cells were washed twice and placed into KRB buffer (132.2 mM NaCl, 3.6 mM KCl, 5 mM NaHCO 3 , 0.5 mM NaH 2 PO 4 , 0.5 mM MgCl 2 , 1.5 mM CaCl 2 and 0.001 g/mL BSA, pH 7.4) supplemented with 25 mM glucose (1 h, 37 C, 5% CO 2 )

Vitamin B12, also known as cobalamin, is a water-soluble vitamin that is naturally found in animal products, including meat, fish, and dairy products, and it is also available as a dietary supplement, an injectable (commonly known as vitamin B12 shots), and in fortified foods

Other research has shown that omega-3s improve nerve structure in diabetes, and they are considered safe at daily doses of up to 3 grams of combined EPA and DHA

Why storage matters Three things are at stake when B12 is stored correctly: Potency