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LCMS metabolite quantification in HeLa cells A total of 1 10 6 treated HeLa cells or 1 10 6 Cas9-expressing HeLa cells were collected, washed with cold PBS and metabolic activity quenched by freezing samples in dry ice and ethanol, and stored at 80 C

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This enzyme takes L-arginine as its direct substrate and converts it into NO plus L-citrulline as a byproduct

Therefore, in the present study, we examined the relative contributions of eNOS to gastric ulcer healing after BPC 157 treatment in rats and, as expected, the AKT phosphorylation, p38/MAPK dephosphorylation and the phosphorylated eNOS expression was increased significantly compared to Clopidogrel group