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2.8 Immunoblot analysis Total protein was isolated from PIG1 and PIG3V cells disrupted in RIPA lysis buffer (Beyotime, China) with protease and phosphatase inhibitor cocktails, and quantified via BCA assay (Beyotime, China)

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Notes: Store in a cool, dry place, out of reach of children

For the latter, patients with a history of bilateral cryptorchidism are at increased risk for infertility

DHT treatment significantly increased MDA levels while decreasing SOD and GSH-PX activities, and YTHDF1 knockdown effectively reversed these changes (Fig