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Insulin (Rat/Mouse Insulin ELISA Kit, Merck-Millipore) and GLP-1 (Glucagon Quantikine ELISA Kit, R&D Systems) in the serum were measured using an enzyme-linked immunosorbent assay

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The His tag (typically 6His, ~0.8 kDa) is the most widely used affinity purification tag, offering simple Ni+/Co+ affinity chromatography with low cost and minimal impact on target protein structure

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Thats why therapies like KPV peptide are so promising