Briefly, the Plugin segments individual nuclei, filters them by size, excludes nuclei touching the edges of the field of view, segments single cells using background subtraction and seeded watershed segmentation, and detects lysosomes using the Laplacian of Gauss (LoG) filter and the Find Maxima command
Neuropeptides 113:102547
However, they were negative for NF, E-cadherin, and Claudin-1 (Figure 6)
A protein shake for breakfast, a small chicken and rice meal for lunch, and a light soup for dinner is a reasonable first-day approach
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