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a) Estimated domain probabilities for the wild-type proline variant

Cells were then lysed in 1X TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.4) supplemented with 1 mM EDTA, 1% Triton X-100, phosphatase inhibitor cocktail (P5726, Sigma Aldrich), and protease inhibitor cocktail (11873580001, Roche Diagnostics)

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