Perfusion experiments were performed with pancreases from both wild-type C57BL6/J mice (Taconic, Denmark), or mice with DT-induced knockdown of proglucagon-producing cells (DT-Gcg) which received i.p
Alignments were then manually optimized with the rationale to avoid insertion/deletion within trans-membrane fragments, but rather move them into loops, and to anchor the beginning or the end of TM helices on special residues, common to the two proteins, like for instance, positively charged residues inside, proline to introduce kinks in helices, etc
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