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However, systemically injected labeled GLP1RAs show limited brain penetration, with the strongest labeling observed in circumventricular organs such as the AP and arcuate nucleus (ARC), suggesting that most central effects are mediated by downstream signaling from these primary GLP1RA binding sites 14

This involves preparing samples and standards in a specific diluent, followed by incubation, washing, and detection steps that culminate in absorbance measurement at designated wavelengths